Multilocus sequence typing of the goose pathogen Mycoplasma anserisalpingitidis

Mycoplasma anserisalpingitidis infection is associated with the inflammation of genital tract and cloaca, embryo lethality, decreased egg production in geese, leading to serious economic losses. M. has been detected mainly Central Eastern Europe, especially Hungary, but pathogen was identified recently China, predicting it’s worldwide occurrence. In this study, a novel multilocus sequence typing (MLST) scheme developed analyse phylogenetic relationships between field isolates clinical specimens originating from different geographical locations. Five loci (atpG, fusA, pgiB, plsY, uvrA) were selected for final MLST study. The examined 89 samples yielded 76 unique types 0.994 Simpson’s index diversity. originated Poland, Ukraine, Vietnam. Phylogenetic analysis revealed existence three distinct clades (A–C) six subclades within clade C. Generally, same locations or livestock integration clustered together. Isolates A showed closest anatis outgroup due similarity plsY locus. highest genetic distance observed 5C among C, containing Asian some Hungarian isolates. assay high diversity investigated samples. method proved be valuable cost-effective tool waterfowl species, enabling better understanding its phylogeny providing robust future molecular epidemiological investigations.